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dna rna damage antibody  (Novus Biologicals)


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    Structured Review

    Novus Biologicals dna rna damage antibody
    Dna Rna Damage Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+rna+damage+antibody/DNA%2FRNA+Damage+Antibody+(15A3)/us12508255-613-0-7
    Average 93 stars, based on 29 article reviews
    dna rna damage antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Development of a model for studying the developmental consequences of oxidative sperm DNA damage by targeting redox-cycling naphthoquinones to the Sertoli cell population.
    Article Snippet: .. Sperm DNA oxidation was determined by immunohistochemistry using a DNA/RNA Damage Antibody that detects 8-OHdG (Novus Biologicals # NB110-96878). ..

    In Situ:

    Article Title: A novel pathway for the induction of DNA damage in human spermatozoa involving extracellular cell-free DNA.
    Article Snippet: DNA damage is a common feature of human spermatozoa associated with an impaired capacity to fertilize the oocyte and an increased mutational load in the offspring.. However, the etiology of this damage remains poorly defined.. In this study we demonstrate that a major pathway for the induction of DNA damage in mammalian spermatozoa is triggered by exposure to exogenous cell free DNA (cfDNA).

    Staining:

    Article Title: A novel pathway for the induction of DNA damage in human spermatozoa involving extracellular cell-free DNA.
    Article Snippet: DNA damage is a common feature of human spermatozoa associated with an impaired capacity to fertilize the oocyte and an increased mutational load in the offspring.. However, the etiology of this damage remains poorly defined.. In this study we demonstrate that a major pathway for the induction of DNA damage in mammalian spermatozoa is triggered by exposure to exogenous cell free DNA (cfDNA).

    Incubation:

    Article Title: Sperm oxidative damage acquired during seminal plasma removal for assisted reproductive technology is reduced by BGP-15
    Article Snippet: .. Cells were fixed in 4% paraformaldehyde for 15 min at 4 °C, then antigen blocked using 1.5% goat serum/PBS for 1 h at room temperature and incubated after with DNA/RNA Damage Antibody (Novus Biologicals) (Littleton, CO, USA) overnight at 4 °C. .. Secondary antibody used was Alexa Fluor 488 Goat anti-Mouse IgG (Life Technologies).

    Article Title: Drinking water quality impacts oocyte viability and embryo development
    Article Snippet: .. Cells were re-pelleted and resuspended into 1:50 DNA/RNA damage antibody (Novus Biologicals, NB110-96878) in PBS and incubated overnight at 4°C. ..

    Article Title: Sperm oxidative damage acquired during seminal plasma removal for assisted reproductive technology is reduced by BGP-15.
    Article Snippet: .. Cells were fixed in 4% paraformaldehyde for 15 min at 4 °C, then antigen blocked using 1.5% goat serum/PBS for 1 h at room temperature and incubated after with DNA/RNA Damage Antibody (Novus Biologicals) (Littleton, CO, USA) overnight at 4 °C. .. Secondary antibody used was Alexa Fluor 488 Goat anti-Mouse IgG (Life Technologies).

    Article Title: Methods and products for improving sperm quality
    Article Snippet: .. Cells were fixed in 4% paraformaldehyde for 15 minutes at 4° C. Cells were then blocked using 1.5% goat serum/PBS for 1 hour at room temperature and then incubated with DNA/RNA Damage Antibody (Novus Biologicals, NB110-96878) overnight at 4° C. Secondary antibody was Goat a Mouse coupled to Alexa Fluor 488 dye. ..

    Article Title: Sub-chronic elevation in ambient temperature drives alterations to the sperm epigenome and accelerates early embryonic development in mice
    Article Snippet: .. Following this, primary antibody incubation was performed with DNA/RNA damage antibody (8-OHdG; Novus, Littleton, CO, USA) or cleaved caspase-3 (Abcam, Cambridge, UK) (both at 5 μg/mL) overnight at 4°C. ..



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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
    Anti 8ohdg Antibody Hy P81140, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+rna+damage+antibody/8-OHdG+(DNA%2FRNA+Damage)+Antibody/pmc12969124-21-0-5
    Average 94 stars, based on 1 article reviews
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    Novus Biologicals dna rna damage antibody
    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
    Dna Rna Damage Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. (H) 8-OHdG levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Journal: iScience

    Article Title: Aluminum exposure impairs nuclear envelope breakdown for mouse zygote formation

    doi: 10.1016/j.isci.2026.115807

    Figure Lengend Snippet: Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. (H) 8-OHdG levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Article Snippet: 8-OHdG antibody , MedChemExpress , Cat# HY- P81140 ; RRID: AB_3103069.

    Techniques: Control, Immunofluorescence, Staining, Fluorescence, Expressing